Methods of testing for food micro-organisms (culture-based methods)
Unveiling the Truth About Standard Plate Counting: A Crucial Tool in Food Safety Management

In this detailed article, we revisit the significance of Standard Plate Counting (SPC), a fundamental microbiological test integral to food safety practices. Often referred to as Mesophilic Count or Aerobic Plate Count, SPC measures bacteria in food samples that grow aerobically at mesophilic temperatures. However, it's crucial to acknowledge its limitations, such as not detecting certain microbial groups like anaerobes or microaerophiles. This exploration discusses the intricacies of SPC, its application under various international standards, and its role not as a definitive indicator of food poisoning risk but as a vital tool in assessing the sanitary handling and temperature management of food products.

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Methods of testing for food micro-organisms (culture-based methods)
Standard Plate Count: A Beginner's Guide to Measuring Bacteria in Food Microbiology

In this article, we're taking you on a delightful dive into the world of food microbiology, specifically geared towards those just starting out. We won't be diving into the nitty-gritty of a full-blown experimental manual here. Instead, think of this as your friendly illustrated guide to understanding how bacteria are measured using the "standard plate count" method.

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Microbial growth conditions and control
A Natural Antimicrobial Agent Transforming Food Preservation and Medicine

In our ongoing exploration of food preservatives, we delve into the fascinating world of nisin, a natural antimicrobial agent that has carved a niche for itself in the global food industry. Originally discovered in fermented milk in 1928, nisin has been celebrated for its effectiveness and safety, receiving approval from various health authorities worldwide, including the FAO/WHO and the U.S. FDA. This article examines nisin’s journey from a humble natural discovery to a cornerstone in food preservation, highlighting its unique properties, mechanisms of action, and its emerging significance in the medical field against antibiotic-resistant bacteria. Join us as we uncover how nisin, a polycationic peptide and a member of the bacteriocins family, continues to make significant contributions to both food safety and human health, proving itself as a vital tool in our antimicrobial arsenal.

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Microbial growth conditions and control
How Glycine Extends the Microbiological Shelf Life of Japanese Convenience Foods

In Japanese convenience stores and supermarkets, the simple amino acid glycine plays a pivotal role in extending the shelf life of popular foods like bento boxes, side dishes, and bakery sweets. This blog post will explore the antibacterial properties of glycine, its unique function in preventing microbial growth, particularly against bacteria forming heat-resistant spores, and its contribution to the flavor profiles of various foods. Dive into the science behind glycine, understanding why this ingredient is so crucial in keeping your favourite convenience foods both safe and delicious.

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Microbial growth conditions and control
From Food Preservation to Antibiotic Alternatives: The Antimicrobial Power of Polylysine and Protamine

Polylysine and protamine, polypeptides comprising 25 to 30 L-lysine and arginine amino acids respectively, are transforming the landscape of microbial inhibition. Initially researched in Japan during the late 1970s for their food preservation properties, these polycations have been pivotal in combating both Gram-negative and Gram-positive bacteria. Their profound impact extends beyond the culinary world into the medical field, where they are now being explored as promising alternatives to conventional antibiotics amidst rising antibiotic resistance.

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Microbial growth conditions and control
Water Activity Explained: What is It and How Does it Differ from Water Content?

In this article, we'll dive into the intriguing world of water activity, cleverly deciphering its principles and how it differs from mere water content. We'll explore the nuances of bound and free water, the principles behind measuring water activity in food, and how reducing water activity can enhance the shelf life of preserved foods like those cured with salt. Additionally, we'll discuss the relationship between water activity and the growth of microbes such as bacteria and mold. Plus, I'll toss in some handy values of water activity to remember that could be a lifesaver—or at least a food saver! So, buckle up, and let's get our science on!

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Non-heat sterilisation of food products
Comparative Analysis of Minimum Infectious Dose and Gastric Acid Resistance Between Salmonella and Enterohemorrhagic Escherichia coli

In this article, we'll explore the differing amounts of bacteria needed to cause food poisoning by two culprits: Salmonella and enterohemorrhagic Escherichia coli. Additionally, we'll discuss a major factor contributing to these differences: the varying resistance to stomach acid between the two.

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Microbial growth conditions and control
How pH Influences Microbial Growth and Organic Acids as Antimicrobials

 In this riveting exploration, we dive into how pH affects the growth and demise of microbes, using sodium acetate, propionic acid, and sorbic acid as our guides to elucidate the mechanisms of antimicrobial action of organic acids. Additionally, we discuss the crucial role of stomach acid in annihilating infectious food poisoning bacteria, highlighting its germicidal functions. Furthermore, we'll explore how variations in acid resistance among food poisoning bacteria relate to the minimum number of bacteria required to cause disease. Lastly, it's fascinating to note that food poisoning bacteria are generally vulnerable to acids, a characteristic intrinsic to their very nature as pathogens.

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Microbial growth conditions and control
What Happens When Bacteria Are Frozen? Do They Die or Survive?

Explore the surprising effects of freezing on bacteria in this scientific inquiry. We examine how microorganisms from different environments respond to extreme cold, focusing on their survival, dormancy, and death mechanisms. This article highlights crucial findings relevant to food safety, revealing how common pathogens like Escherichia coli O157 react under freezing conditions. Dive into the fascinating interplay between microbiology and freezing temperatures, and discover what this means for food hygiene practices.

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Microbial growth conditions and control
Microbial Growth and Temperature: Key Insights for Food Safety

Understanding how microbes interact with temperature is essential for food safety. While many foodborne pathogens, such as mesophiles, thrive at moderate temperatures, there is a common misconception that psychrophiles—microbes that adapt to cold conditions—only grow in fridge-like environments. This article demystifies the growth behaviors of these temperature-sensitive bacteria, including the versatile Listeria monocytogenes, and explores the biological adaptations that allow certain microbes to thrive in colder climates. Strengthen your knowledge of microbiological principles critical to ensuring food safety.

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